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Proceedings Paper

In vivo multiphoton and second harmonic generation microscopy of epithelial carcinogenesis
Author(s): Gracie Vargas; Tuya Shilagard; Ju Sun; Massoud Motamedi
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Paper Abstract

Multiphoton microscopy and second harmonic generation microscopy were used to image epithelial changes in a hamster model for oral malignant transformation. In vivo imaging was performed to characterize morphometric alterations in normal and precancerous regions. Morphometric measurements such as cell nucleus area and epithelial thicknesses obtained from MPM-SHGM were in excellent agreement with histology obtained after in vivo imaging. MPM-SHGM was highly sensitive to spectroscopic and architectural alterations throughout carcinogenesis, showing statistically significant changes in morphology. MPM revealed hyperkeratosis, nuclear enlargement/crowding in dysplasia, and immune cell infiltration. SHGM revealed alterations in submucosal architecture, with a decrease in SHG density evident during early stages of precancer. By combining MPM with SHGM, the basement membrane could be identified in normal, hyperplasia, and dysplasia samples and in some cases of early invasion. The combined technique of MPM-SHGM has the potential to serve as an adjunct to biopsy for assessing precancerous changes and will be investigated further for that purpose. Additionally, the method can provide spatiotemporal assessment of early neoplastic changes in order to elucidate the stages of transformation in vivo and could be used to assess therapeutic efficacy of agents being tested for the treatment of epithelial precancers/cancer.

Paper Details

Date Published: 22 February 2006
PDF: 8 pages
Proc. SPIE 6091, Optical Biopsy VI, 60910B (22 February 2006); doi: 10.1117/12.646564
Show Author Affiliations
Gracie Vargas, The Univ. of Texas Medical Branch (United States)
Tuya Shilagard, The Univ. of Texas Medical Branch (United States)
Ju Sun, Florida International Univ. (United States)
Massoud Motamedi, The Univ. of Texas Medical Branch (United States)

Published in SPIE Proceedings Vol. 6091:
Optical Biopsy VI
Robert R. Alfano; Alvin Katz, Editor(s)

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