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Proceedings Paper

Elastic scattering spectroscopy of coagulated brain tissues
Author(s): Filiz Ateş; Haşim Özgür Tabakoğlu; Özgüncem Bozkulak; Murat Canpolat; Murat Gülsoy
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Paper Abstract

The goal of this study was to differentiate the parts of lamb brain according to elastic scattering spectroscopy and detect the optical alterations due to coagulation. Cells and tissues are not uniform and have complex structures and shapes. They can be referred to as scattering particles. The process of scattering depends on the light wavelength and on the scattering medium properties; especially on the size and the density of the medium. When elastic scattering spectroscopy (ESS) is employed, the morphological alterations of tissues can be detected using spectral measurements of the elastic scattered light over a wide range of wavelengths. In this study firstly, the slopes of ESS spectra were used to differentiate the parts of lamb brains (brainstem, cerebellum, gray matter, white matter) in vitro in the range of 450 - 750 nm. Secondly, tissues were coagulated at different temperatures (45, 60, and 80 °C) and ESS spectra were taken from native and coagulated tissues. It was observed that as the coagulation temperature increased, the slope of the elastic scattering spectra decreased. Thus, optical properties of tissues were changed with respect to the change in nuclear to cytoplasmic ratio due to the water loss. Results showed that the slopes of ESS spectra in the visible range revealed valuable information about the morphological changes caused by coagulation.

Paper Details

Date Published: 28 February 2006
PDF: 7 pages
Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60840Q (28 February 2006); doi: 10.1117/12.646195
Show Author Affiliations
Filiz Ateş, Boğaziçi Univ. (Turkey)
Haşim Özgür Tabakoğlu, Boğaziçi Univ. (Turkey)
Özgüncem Bozkulak, Boğaziçi Univ. (Turkey)
Murat Canpolat, Medical School of Akdeniz Univ. (Turkey)
Murat Gülsoy, Boğaziçi Univ. (Turkey)

Published in SPIE Proceedings Vol. 6084:
Optical Interactions with Tissue and Cells XVII
Steven L. Jacques; William P. Roach, Editor(s)

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