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Proceedings Paper

Chlorin p6 as a fluorescent probe for the investigation of surfactant-cyclodextrin interactions
Author(s): Padmaja P Mishra; Ramkrishna Adhikary; Priyanka Lahiri; Anindya Datta
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Paper Abstract

Cyclodextrins (CD) are often projected as potential vehicles in targeted drug delivery. However, if the membrane structure is disrupted by CD, then it cannot be considered to be a good drug delivery vehicle. When an extrinsic fluorescence probe is used to monitor such interactions, there are no less than three possible equilibria that can operate simultaneously: surfactant-cyclodextrin, surfactant-fluorophore and cyclodextrin-fluorophore. The fluorescence intensity / lifetime might be affected by all these and so, the results depend strongly on the fluorophore used as well as the nature of the surfactant. This aspect highlights the importance of the suitability of the fluorescence probe to be used to study complicated systems and interaction. In the present work, chlorin p6, prepared from chlorophyll from spinach leaves, has been used as the fluorescence probe to investigate the interaction between α-CD and β-CD with the neutral surfactants TX 100 and CTAB. The fluorophore is found to be a sensitive one for the study of the interaction of α- and β-CD with the surfactants Tritron X 100 (TX 100) and cetyl trimethyl ammonium bromide (CTAB). It is found that contrary to earlier reports, a complex between α-CD and TX 100 is formed, even though the binding constant is not very high. This observation can be obtained with chlorin p6, which does not bind to the CDs, but not with a fluorophore like TNS, which binds to the CD as well and thus complicates the situation as the binding with CD is stronger than that between TX 100 and α-CD as compared to that between TNS and CD.

Paper Details

Date Published: 10 February 2006
PDF: 10 pages
Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications, 609708 (10 February 2006); doi: 10.1117/12.645958
Show Author Affiliations
Padmaja P Mishra, Indian Institute of Technology Bombay (India)
Ramkrishna Adhikary, Indian Institute of Technology Bombay (India)
Priyanka Lahiri, Indian Institute of Technology Bombay (India)
Anindya Datta, Indian Institute of Technology Bombay (India)

Published in SPIE Proceedings Vol. 6097:
Optical Molecular Probes for Biomedical Applications
Samuel I. Achilefu; Darryl J. Bornhop; Ramesh Raghavachari, Editor(s)

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