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Proceedings Paper

Study of human breast tissues biochemistry by FT-Raman spectroscopy
Author(s): Renata Andrade Bitar; Walter Andres Avendano Jara; Mário M. Netto; Herculano Martinho; Leandra Náira Zambelli Ramalho; Airton Abrahão Martin
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Paper Abstract

In this work we employ the Fourier Transform Raman Spectroscopy to study the human breast tissues, both normal and pathological. In the present study we analyze 194 Raman spectra from breast tissues that were separated into 9 groups according to their corresponding histopathological diagnosis, which are as follows: Normal breast tissue, Fibrocystic condition, In Situ Duct Carcinoma, In Situ Duct Carcinoma with Necrosis, Infiltrating Duct Carcinoma, Infiltrating Duct Inflammatory Carcinoma, Infiltrating Duct Medullar Carcinoma, Infiltrating Duct Colloid Carcinoma, and Infiltrating Lobule Carcinoma. We found a strong lipids Raman band, and this structure was identified as abundant in the normal breast tissue spectra. The primary structure of proteins was identified through the shift of the amine acids bands. The identification of the secondary structure of proteins occurred through the peptide bands (Amide I and Amide III). In relation to the carbohydrates, the spectra of duct infiltrating colloid carcinoma, fibrocystic condition, and infiltrating duct carcinoma have been compared and identified. We observed an increase in the intensity of the 800-1200 cm-1 spectral region. This fact could indicate the presence of liquid cystic. We also notice alterations in the peaks in the region of 500 to 600 cm-1 and 2000 to 2100 cm-1 that may suggest changes in the nucleic acids of the cells.

Paper Details

Date Published: 27 February 2006
PDF: 10 pages
Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 60930Y (27 February 2006); doi: 10.1117/12.645884
Show Author Affiliations
Renata Andrade Bitar, Univ. of the Valley of Paráiba (Brazil)
Walter Andres Avendano Jara, Univ. of the Valley of Paráiba (Brazil)
Mário M. Netto, A.C. Camargo Hospital - Hospital do Cancer (Brazil)
Herculano Martinho, Univ. of the Valley of Paráiba (Brazil)
Leandra Náira Zambelli Ramalho, Ribeirão Preto Medicine College, Univ. of São Paulo (Brazil)
Airton Abrahão Martin, Univ. of the Valley of Paráiba (Brazil)

Published in SPIE Proceedings Vol. 6093:
Biomedical Vibrational Spectroscopy III: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang H. Petrich, Editor(s)

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