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Proceedings Paper

Nanosurgery of sub-cellular organelles in living cells using a femtosecond laser oscillator
Author(s): Wataru Watanabe; Tomoko Shimada; Sachihiro Matsunaga; Hiroshi Ishii; Tsunehito Higashi; Kiichi Fukui; Kazuyoshi Itoh
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Paper Abstract

Femtosecond laser pulses in the near-infrared region have potential applications in nanosurgery in cell biology. Femtosecond laser pulses can be used to selectively disrupt and dissect intracellular organelles in living cells. We report on disruption of fluorescence-labeled nucleus and mitochondria in living HeLa cells using a femtosecond Ti:sapphire laser oscillator with a repetition rate of 76 MHz. We examined the effectiveness of the restaining method in combination with fluorescence recovery after photobleaching (FRAP) analysis to discern disruption or bleaching.

Paper Details

Date Published: 28 February 2006
PDF: 5 pages
Proc. SPIE 6108, Commercial and Biomedical Applications of Ultrafast Lasers VI, 610804 (28 February 2006); doi: 10.1117/12.645474
Show Author Affiliations
Wataru Watanabe, Osaka Univ. (Japan)
Tomoko Shimada, Osaka Univ. (Japan)
Sachihiro Matsunaga, Osaka Univ. (Japan)
Hiroshi Ishii, Osaka Univ. (Japan)
Tsunehito Higashi, Osaka Univ. (Japan)
Kiichi Fukui, Osaka Univ. (Japan)
Kazuyoshi Itoh, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 6108:
Commercial and Biomedical Applications of Ultrafast Lasers VI
Joseph Neev; Stefan Nolte; Alexander Heisterkamp; Christopher B. Schaffer, Editor(s)

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