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Proceedings Paper

Immune cells tracing using quantum dots
Author(s): Akiyoshi Hoshino; Kouki Fujioka; Yuki I. Kawamura; Noriko Toyama-Sorimachi; Masato Yasuhara M.D.; Taeko Dohi M.D.; Kenji Yamamoto M.D.
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Paper Abstract

Fluorescent nanoparticles, such as nanocrystal quantum dots (QDs), have potential to be applied to molecular biology and bioimaging, since some nanocrystals emit higher and longer lasting fluorescence than conventional organic probes do. Here we report an example of labeling immune cells by QDs. We collected splenic CD4+ T-lymphocyte and peritoneal macrophages from mice. Then cells were labeled with QDs. QDs are incorporated into the T-lymphocyte and macrophages immediately after addition and located in the cytoplasm via endocytosis pathway. The fluorescence of QDs held in the endosomes was easily detected for more than a week. In addition, T-lymphocytes labeled with QDs were stable and cell proliferation or cytokine production including IL-2 and IFN-γ was not affected. When QD-labeled T-lymphocytes were adoptively transferred intravenously to mice, they remained in the peripheral blood and spleen up to a week. Using QD-labeled peritoneal macrophages, we studied cell traffic during inflammation on viscera in peritoneum cavity. QD-labeled macrophages were transplanted into the peritoneum of the mouse, and colitis was induced by intracolonic injection of a hapten, trinitrobenzensulfonic acid. With the aid of stong signals of QDs, we found that macrophage accumuled on the inflammation site of the colon. These results suggested that fluorescent probes of QDs might be useful as bioimaging tools for tracing target cells in vivo.

Paper Details

Date Published: 27 March 2006
PDF: 8 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 609613 (27 March 2006); doi: 10.1117/12.645420
Show Author Affiliations
Akiyoshi Hoshino, International Medical Ctr. of Japan (Japan)
Tokyo Medical and Dental Univ. (Japan)
Kouki Fujioka, International Medical Ctr. of Japan (Japan)
Yuki I. Kawamura, International Medical Ctr. of Japan (Japan)
Noriko Toyama-Sorimachi, International Medical Ctr. of Japan (Japan)
Masato Yasuhara M.D., Tokyo Medical and Dental Univ. (Japan)
Taeko Dohi M.D., International Medical Ctr. of Japan (Japan)
Kenji Yamamoto M.D., International Medical Ctr. of Japan (Japan)
Tokyo Medical and Dental Univ. (Japan)

Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin M.D., Editor(s)

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