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Proceedings Paper

Direct measurements of blood cell density and velocity in retinal microvessels
Author(s): F. Lacombe; M. Glanc; J. Le Gargasson; C. Bellmann; M. Paques; J. Sahel
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Paper Abstract

Adaptive Optics assisted eye fundus imaging now provides high resolution images of the very contents of the retinal tissue. Impressive images of the mosaic of the photoreceptors have obtained by several groups and the best reported images even show the circulatory activity in capillars. Nevertheless, the natural eye movements, such as drifts or micro-saccades, generally prevent from an accurate registration of the field of view. Moreover, images obtained through scanning techniques also suffer from field distorsion. These two effects make it difficult to unambiguously extract blood cell density and velocity information. We report how Adaptive Optics assisted flood imaging of the retina, associated with an original a posteriori image registration and data analysis now permits evaluating and mapping the flow activity in the retinal micro-vessels. Examples where capillars as thin as a few micrometers are revealed only by their circulatory activity will be given. Larger field of view along the retinal vascular network will also be produced. Thanks to this high resolution functional mapping, we'll demonstrate how blood cells direct observation can be enhanced and how quantitative parameters such as velocity or density can be derived. Velocity maps of the macular vascular network at the eye's diffraction limit will finally be produced.

Paper Details

Date Published: 13 February 2006
PDF: 11 pages
Proc. SPIE 6138, Ophthalmic Technologies XVI, 61381M (13 February 2006); doi: 10.1117/12.645241
Show Author Affiliations
F. Lacombe, Mauna Kea Technologies (France)
M. Glanc, LESIA, Observatoire de Paris (France)
J. Le Gargasson, INSERM (France)
C. Bellmann, INSERM (France)
M. Paques, INSERM (France)
J. Sahel, INSERM (France)

Published in SPIE Proceedings Vol. 6138:
Ophthalmic Technologies XVI
Fabrice Manns; Per G. Söderberg; Arthur Ho, Editor(s)

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