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Proceedings Paper

Locked nucleic acid molecular beacons for intracellular mRNA monitoring
Author(s): Lin Wang; Chaoyong James Yang; Colin D. Medley; Steven A. Benner; Weihong Tan
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Paper Abstract

We have designed, synthesized, and investigated a novel molecular beacon (MB) using locked nucleic acid (LNA) bases for intracellular mRNA monitoring. This new LNA-MB has several useful properties including: very high melting temperature; excellent affinity for complementary sequences; superior single base mismatch discrimination capability; stablity against nuclease digestion; and not binding with single-stranded DNA binding proteins. All of these properties are highly advantageous for a molecular tool for various intracellular studies of biochemical, biological and medical significance.

Paper Details

Date Published: 28 February 2006
PDF: 14 pages
Proc. SPIE 6095, Nanobiophotonics and Biomedical Applications III, 60950M (28 February 2006); doi: 10.1117/12.644649
Show Author Affiliations
Lin Wang, Univ. of Florida (United States)
Chaoyong James Yang, Univ. of Florida (United States)
Colin D. Medley, Univ. of Florida (United States)
Steven A. Benner, Univ. of Florida (United States)
Weihong Tan, Univ. of Florida (United States)

Published in SPIE Proceedings Vol. 6095:
Nanobiophotonics and Biomedical Applications III
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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