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Proceedings Paper

Dynamic imaging of interaction between protein 14-3-3 and Bid in living cells
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Paper Abstract

The 14-3-3 proteins are known to sequester certain pro-apoptotic members of this family. BH3- interacting domain death agonist (Bid) may contribute to tumor necrosis factor α(TNF-α)-induced neuronal death, although regulation by 14-3-3 has not been reported. In this study we examined whether 14-3-3 proteins interact with Bid/tBid during TNF-α-induced cell death. The TNF-αtriggered Bid cleavage and tBid translocated to mitochondria. Human lung adenocarcinoma cells were co-transfected with both CFP-Bid and 14-3-3-YFP plasmids, and the dynamical interaction between the Bid/tBid and 14-3-3 were performed on laser confocal fluorescence microscope in single living cell during TNF-α-induced cell apoptosis. The Bid distribute equally only in the cytoplasm of healthy cells, and the 14-3-3 protein distribute not only in the cytoplasm but also in the nucleus of healthy cells. Our data showed that the tBid aggregate, but the 14-3-3 protein does not aggregate as the tBid, and the 14-3-3 protein separate from the aggregated tBid, implying that the 14-3-3 proteins do not interact with the aggregated tBid after TNF-αtreatment.

Paper Details

Date Published: 24 February 2006
PDF: 10 pages
Proc. SPIE 6087, Biophotonics and Immune Responses, 60870L (24 February 2006); doi: 10.1117/12.644517
Show Author Affiliations
Tongsheng Chen, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Jinjun Wang, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 6087:
Biophotonics and Immune Responses
Wei R. Chen, Editor(s)

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