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Proceedings Paper

Image correlation method for measuring flow and diameter changes in contracting mesenteric microlymphatics in situ
Author(s): J. Brandon Dixon; Gerard Cote; Anatoly Gashev; Steven Greiner; James Moore; David Zawieja
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Paper Abstract

Collecting microlymphatics play a vital role in promoting lymph flow from the initial lymphatics in the interstitial spaces to the large transport lymph ducts. In most tissues, the primary mechanism for producing this flow is the spontaneous contractions of the lymphatic wall. Individual units, known as lymphangion, are separated by valves that help prevent backflow when the vessel contracts, thus promoting flow through the lymphatic network. Lymphatic contractile activity is inhibited by flow in isolated lymphatics, however there are virtually no in situ measurements of lymph flow in these vessels. One of the difficulties associated with obtaining such measurements is the time consuming methods of manual particle tracking used previously by our group. Using an in situ preparation with mesenteric microlymphatics (~ 100 μm in diameter) and a high speed imaging system (500 fps), we have developed an image correlation method to measure lymphatic flow with a standard error of prediction of 0.3 mm/sec when compared with manual particle tracking.

Paper Details

Date Published: 21 February 2006
PDF: 4 pages
Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60880W (21 February 2006); doi: 10.1117/12.644290
Show Author Affiliations
J. Brandon Dixon, Texas A&M Univ. (United States)
Gerard Cote, Texas A&M Univ. (United States)
Anatoly Gashev, Texas A&M Univ. Health Science Ctr. (United States)
Steven Greiner, Texas A&M Univ. Health Science Ctr. (United States)
James Moore, Texas A&M Univ. (United States)
David Zawieja, Texas A&M Univ. Health Science Ctr. (United States)

Published in SPIE Proceedings Vol. 6088:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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