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Proceedings Paper

Surface enhanced Raman spectroscopy (SERS) from a molecule adsorbed on a nanoscale silver particle cluster in a holographic plate
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Paper Abstract

Surface enhanced Raman spectroscopy has become a viable technique for the detection of single molecules. This highly sensitive technique is due to the very large (up to 14 orders in magnitude) enhancement in the Raman cross section when the molecule is adsorbed on a metal nanoparticle cluster. We report here SERS (Surface Enhanced Raman Spectroscopy) experiments performed by adsorbing analyte molecules on nanoscale silver particle clusters within the gelatin layer of commercially available holographic plates which have been developed and fixed. The Ag particles range in size between 5 - 30 nanometers (nm). Sample preparation was performed by immersing the prepared holographic plate in an analyte solution for a few minutes. We report here the production of SERS signals from Rhodamine 6G (R6G) molecules of nanomolar concentration. These measurements demonstrate a fast, low cost, reproducible technique of producing SERS substrates in a matter of minutes compared to the conventional procedure of preparing Ag clusters from colloidal solutions. SERS active colloidal solutions require up to a full day to prepare. In addition, the preparations of colloidal aggregates are not consistent in shape, contain additional interfering chemicals, and do not generate consistent SERS enhancement. Colloidal solutions require the addition of KCl or NaCl to increase the ionic strength to allow aggregation and cluster formation. We find no need to add KCl or NaCl to create SERS active clusters in the holographic gelatin matrix. These holographic plates, prepared using simple, conventional procedures, can be stored in an inert environment and preserve SERS activity after several weeks subsequent to preparation.

Paper Details

Date Published: 23 February 2006
PDF: 7 pages
Proc. SPIE 6099, Plasmonics in Biology and Medicine III, 609901 (23 February 2006); doi: 10.1117/12.643177
Show Author Affiliations
Leonard E. Jusinski, Sandia National Labs. (United States)
Ramen Bahuguna, San Jose State Univ. (United States)
Amrita Das, San Jose State Univ. (United States)
Karamjeet Arya, San Jose State Univ. (United States)

Published in SPIE Proceedings Vol. 6099:
Plasmonics in Biology and Medicine III
Tuan Vo-Dinh; Joseph R. Lakowicz; Zygmunt Gryczynski, Editor(s)

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