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Proceedings Paper

Whole-cell luminescence biosensor-based lab-on-chip integrated system for water toxicity analysis
Author(s): Arthur Rabner; Shimshon Belkin; Rachel Rozen; Yosi Shacham
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Paper Abstract

A novel water chemical toxin sensor has been successfully developed and evaluated as a working portable laboratory prototype. This sensor relies on a disposable plastic biochip prepared with a 4x4 micro-laboratory (μLab) chambers array of Escherichia coli reporter cells and micro-fluidic channels for liquids translocation. Each bacterial strain has been genetically modified into a bioluminescent reporter that responds to a pre-determined class of chemical agents. When challenged with a water sample containing a toxic chemical, the sensor responds with an increased bioluminescent signal from the biochip that is monitored over time. The signal is received by a motorized photomultiplier-based analyzer and interpreted by signal processing software. We have performed several levels of analysis: (i) the change in the bioluminescent signal from the sensor bacteria serves as a rapid indication for the presence of toxic chemicals in the water sample; (ii) the intensity of the change indicates the toxin concentration level; and (iii) the pattern of the responses for the different members of the bacterial panel on the biochip characterizes the biological origin of the toxin. The analyzer contains housing mechanics, electro-optics for signal acquisition, motorized readout calibration accessories, hydro-pneumatics modules for water sample translocation into biochip micro laboratories, electronics for overall control and communication with the host computer. This prototype has a demonstrated sensitivity for broad classes of water-borne toxic chemicals including naladixic acid (a model genotoxic agent), botulinum and acetylcholine esterase inhibitors. This work has initiated an investigation of a novel handheld field-deployable Water Toxicity Analysis (WTA) device.

Paper Details

Date Published: 23 January 2006
PDF: 10 pages
Proc. SPIE 6112, Microfluidics, BioMEMS, and Medical Microsystems IV, 611205 (23 January 2006); doi: 10.1117/12.640679
Show Author Affiliations
Arthur Rabner, Tel-Aviv Univ. (Israel)
Shimshon Belkin, The Hebrew Univ. (Israel)
Rachel Rozen, The Hebrew Univ. of Jerusalem (Israel)
Yosi Shacham, Tel-Aviv Univ. (Israel)

Published in SPIE Proceedings Vol. 6112:
Microfluidics, BioMEMS, and Medical Microsystems IV
Ian Papautsky; Wanjun Wang, Editor(s)

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