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Proceedings Paper

Optical coherence tomography imaging and fluorescence spectroscopy of a novel rat model of ovarian cancer
Author(s): Elizabeth Kanter; Ross Walker; Sam Marion; Patricia Hoyer; Jennifer K. Barton
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Paper Abstract

Ovarian cancer is relatively rare but is the fifth leading cause of death from cancer in women. Little is known about the precursors and early stages of ovarian cancer partially due to the lack of a realistic animal model. A cohesive model that incorporates ovarian cancer induction into a menopausal rodent would be well suited for comprehensive studies of ovarian cancer, and non-destructive imaging would allow carcinogenesis to be followed. Optical Coherence Tomography (OCT) and Light-Induced Fluorescence (LIF) are minimally invasive optical modalities that allow both structural and biochemical changes to be noted. Rat ovaries were exposed to 4-vinylcyclohexene diepoxide (VCD) for 20 days in order to destroy the primordial follicles. Sutures coated with 7,12-dimethylbenz(a)anthracene (DMBA) were implanted in the right ovary, in order to produce epithelial based ovarian cancers. Rats were sacrificed at 1, 3, and 5 months and ovaries were harvested and imaged with a combined OCT/LIF system. Histology was preformed on the harvested ovaries and any pathology determined. OCT was able to visualize follicle loss and DMBA-induced abnormalities. LIF spectra were also different between cycling, follicle deplete, and DMBA-exposed ovaries. Overall this pilot study demonstrated the feasibility of both the animal model and optical imaging.

Paper Details

Date Published: 7 October 2005
PDF: 8 pages
Proc. SPIE 5861, Optical Coherence Tomography and Coherence Techniques II, 58610P (7 October 2005); doi: 10.1117/12.632983
Show Author Affiliations
Elizabeth Kanter, The Univ. of Arizona (United States)
Ross Walker, The Univ. of Arizona (United States)
Sam Marion, The Univ. of Arizona (United States)
Patricia Hoyer, The Univ. of Arizona (United States)
Jennifer K. Barton, The Univ. of Arizona (United States)

Published in SPIE Proceedings Vol. 5861:
Optical Coherence Tomography and Coherence Techniques II
Wolfgang Drexler, Editor(s)

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