A standard method in confocal microscopy to form an extended focus image is to merely add together (integrate) a number of optical sections taken throughout the specimen volume of interest. If we use this method in a conventional microscope the image that results is of rather poor quality. However since the image has been degraded in a known fashion and it is straightforward, by using simple inverse filtering techniques, to restore a high quality extended depth of focus image. Examples will be shown obtained in both the fluorescence and brightfield imaging modes. The method is also suited to high resolution stereo imaging.

Date Published: 7 October 2005
PDF: 6 pages
Proc. SPIE 5860, Confocal, Multiphoton, and Nonlinear Microscopic Imaging II, 58600H (7 October 2005); doi: 10.1117/12.632947

Published in SPIE Proceedings Vol. 5860:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging II
Tony Wilson, Editor(s)