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Proceedings Paper

Nanomaterials: hammerheading a new frontier
Author(s): T. L. Jennings; J. C. Schlatterer; N. L. Greenbaum; G. F. Strouse
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Paper Abstract

Energy transfer from organic fluorophores to small metal nanoparticles is being used as a molecular beacon tool to monitor the kinetic processes of the hammerhead ribozyme. This marks the first time that nanomaterials have been used to monitor ribozyme kinetics. The quantum efficiency of energy transfer from the fluorophore to the gold nanoparticle follows a distance dependence behavior, which allows the real-time characterization of ribozyme complex structure and cleavage kinetics. The rate of cleavage for our ribozyme at pH=6.5 and 37°C is measured to be on the order of 10-2 min-1, which is the correct order of magnitude for similar ribozymes at this pH in the literature.

Paper Details

Date Published: 21 November 2005
PDF: 8 pages
Proc. SPIE 6009, Optical Methods in Drug Discovery and Development, 60090O (21 November 2005); doi: 10.1117/12.631418
Show Author Affiliations
T. L. Jennings, Florida State Univ. (United States)
J. C. Schlatterer, Florida State Univ. (United States)
N. L. Greenbaum, Florida State Univ. (United States)
G. F. Strouse, Florida State Univ. (United States)

Published in SPIE Proceedings Vol. 6009:
Optical Methods in Drug Discovery and Development
Mostafa Analoui; David A. Dunn, Editor(s)

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