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Proceedings Paper

Microarray immunoassay for phenoxybenzoic acid using polymer-functionalized lanthanide oxide nanoparticles as fluorescent labels
Author(s): Mikaela Nichkova; Dosi Dosev; Shirley J. Gee; Bruce D. Hammock; Ian M. Kennedy
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Paper Abstract

Fluorescent properties and low production cost makes lanthanide oxide nanoparticles attractive labels in biochemistry. Nanoparticles with different fluorescent spectra were produced by doping of oxides such as Y2O3 and Gd2O3 with different lanthanide ions (Eu, Tb, Sm) giving the possibility for multicolor labeling. Protein microarrays have the potential to play a fundamental role in the miniaturization of biosensors, clinical immunological assays, and protein-protein interaction studies. Here we present the application of fluorescent lanthanide oxide nanoparticles as labels in microarray-based immunoassay for phenoxybenzoic acid (PBA), a generic biomarker of human exposure to the highly potent insecticides pyrethroids. A novel polymer-based protocol was developed for biochemical functionalization of the nanoparticles. Microarrays of antibodies were fabricated by microcontact printing in line patterns onto glass substrates and immunoassays were successfully performed using the corresponding functionalized nanoparticles. The applicability of the fluorophore nanoparticles as reporters for detection of antibody-antigen interactions has been demonstrated for phenoxybenzoic acid (PBA)/anti-PBA IgG. The sensitivity of the competitive fluorescent immunoassay for PBA was similar to that of the corresponding ELISA.

Paper Details

Date Published: 17 November 2005
PDF: 9 pages
Proc. SPIE 6008, Nanosensing: Materials and Devices II, 600815 (17 November 2005); doi: 10.1117/12.631032
Show Author Affiliations
Mikaela Nichkova, Univ. of California, Davis (United States)
Dosi Dosev, Univ. of California, Davis (United States)
Shirley J. Gee, Univ. of California, Davis (United States)
Bruce D. Hammock, Univ. of California, Davis (United States)
Ian M. Kennedy, Univ. of California, Davis (United States)

Published in SPIE Proceedings Vol. 6008:
Nanosensing: Materials and Devices II
M. Saif Islam; Achyut K. Dutta, Editor(s)

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