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Proceedings Paper

Development of an electrochemical biosensor without a sandwich assay
Author(s): James J. Sumner; Kevin W. Plaxco; Carl D. Meinhart; Hyongsok Soh
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Paper Abstract

The combination of electrochemistry with microfluidic sample processing is a viable option to reduce the size, logistics load and power consumption of biosensors. Modern microfluidics technology makes it possible to perform sample clean-up, PCR, sample concentration and transduction on the same disposable chip. This presentation will discuss two novel electrochemical techniques which do not require a sandwich assay and can be employed on a disposable microfluidic chip, reducing logistics load and microfluidic complexity. Transduction is achieved via an electrochemical DNA hybridization sensor similar to a molecular beacon removing the need for a sandwich assay also referred to as E-DNA. The sensor is designed where a DNA stem-loop structure is immobilized on a gold electrode with a redox label held close to the surface. Upon hybridization the stem-loop opens and the label pulls away from the surface so that current cannot flow to the electrode under positive bias. This paper will primarily discuss experiments trying to understand the hybridization event and effect of surface morphology on electrochemical signal transduction.

Paper Details

Date Published: 11 November 2005
PDF: 6 pages
Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 600706 (11 November 2005); doi: 10.1117/12.630748
Show Author Affiliations
James J. Sumner, U.S. Army Research Lab. (United States)
Kevin W. Plaxco, Univ. of California/Santa Barbara (United States)
Carl D. Meinhart, Univ. of California/Santa Barbara (United States)
Hyongsok Soh, Univ. of California/Santa Barbara (United States)

Published in SPIE Proceedings Vol. 6007:
Smart Medical and Biomedical Sensor Technology III
Brian M. Cullum; J. Chance Carter, Editor(s)

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