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Proceedings Paper

Detection of biomolecules in complex media using surface plasmon resonance sensors
Author(s): Michael R. Malone; Jean-Francois Masson; Margaret Barhnart; Stephen Beaudoin; Karl S. Booksh
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Paper Abstract

Detection of multiple biologically relevant molecules was accomplished at sub-ng/mL levels in highly fouling media using fiber- optic based surface plasmon resonance sensors. Myocardial infarction markers, myoglobin and cTnI, were quantified in full serum with limits of detection below 1 ng/mL. Biologically relevant levels are between 15-30 ng/mL and 1-5 ng/mL for myoglobin and cTnI respectively. Cytokines involved in chronic wound healing, Interleukin 1, Interleukin 6, and tumor necrosis factor α, were detected at around 1 ng/mL in cell culture media. Preliminary results in monitoring these cytokines in cell cultures expressing the cytokines were obtained. The protein diagnostic of spinal muscular atrophy, survival motor neuron protein, was quantified from cell lysate. To obtain such results in complex media, the sensor's stability to non-specific protein adsorption had to be optimized. A layer of the N-hydroxysuccinimide ester of 16-mercaptohexadecanoic acid is attached to the sensor. This layer optimizes the antibody attachment to the sensor while minimizing the non-specific signal from serum proteins.

Paper Details

Date Published: 11 November 2005
PDF: 12 pages
Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 60070V (11 November 2005); doi: 10.1117/12.630736
Show Author Affiliations
Michael R. Malone, Arizona State Univ. (United States)
Jean-Francois Masson, Arizona State Univ. (United States)
Margaret Barhnart, Arizona State Univ. (United States)
Stephen Beaudoin, Purdue Univ. (United States)
Karl S. Booksh, Arizona State Univ. (United States)

Published in SPIE Proceedings Vol. 6007:
Smart Medical and Biomedical Sensor Technology III
Brian M. Cullum; J. Chance Carter, Editor(s)

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