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Proceedings Paper

A label-free optical waveguide biosensor with sub-wavelength gratings
Author(s): Jenq-Nan Yih; Yen-Chieh Mao; Yi-Ming Chu; Wei-Han Wang; Lai-Sheng Chen; Fan-Ching Chien; Kuang-Li Lee; Pei-Kuan Wei; Shean-Jen Chen
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Paper Abstract

In this paper, the reflection resonance spectrum of a sub-wavelength diffraction grating-coupled waveguide is used to analyze biomolecular interactions in real time. When the diffraction grating waveguide structure is destroyed by external factors such as slight refractive index changes of the buffer or molecule adsorption on the grating surface, the optical path of the light coupled through the grating into the waveguide is changed and a resonance wavelength shift is induced as a result. By detecting this resonance wavelength shift, the optical waveguide biosensor provides the ability to identify the kinetics of the biomolecular interaction on an on-line basis without the need for the extrinsic labeling of the biomolecules. A theoretical analysis of the sub-wavelength optical waveguide biosensor is performed. A biosensor with a narrow reflection resonance spectrum, and hence an enhanced detection resolution, is then designed and fabricated. Currently, the detection limit of the optical waveguide sensor is found to be approximately 10-5 refractive index units. The developed biosensor is successfully applied to study the kinetics of an antibody interaction with protein G adsorbed on the sensing surface.

Paper Details

Date Published: 11 November 2005
PDF: 9 pages
Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 600712 (11 November 2005); doi: 10.1117/12.630610
Show Author Affiliations
Jenq-Nan Yih, National Central Univ. (Taiwan)
Yen-Chieh Mao, Industrial Technology Research Institute (Taiwan)
Yi-Ming Chu, Industrial Technology Research Institute (Taiwan)
Wei-Han Wang, Industrial Technology Research Institute (Taiwan)
Lai-Sheng Chen, Industrial Technology Research Institute (Taiwan)
Fan-Ching Chien, National Central Univ. (Taiwan)
Kuang-Li Lee, Academia Sinica (Taiwan)
Pei-Kuan Wei, Academia Sinica (Taiwan)
Shean-Jen Chen, National Cheng Kung Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6007:
Smart Medical and Biomedical Sensor Technology III
Brian M. Cullum; J. Chance Carter, Editor(s)

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