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Proceedings Paper

Detection of fluorescence spectra of individual bioaerosol particles
Author(s): Per Jonsson; Fredrik Kullander; Pär Wästerby; Mikael Tiihonen; Mikael Lindgren
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Paper Abstract

We present initial results of a measurement system designed for detecting the fluorescence spectrum of individual particles of biological warfare agent (BWA). A compact optical parametric oscillator with intracavity sum-frequency mixing and a commercial Nitrogen gas laser was used as excitation sources to generate 293 nm or 337 nm UV laser irradiation. The pulsed lasers and a photomultiplier tube (PMT) array based spectrometer were triggered by a red laser-diode and a PMT detector that sensed the presence of a particle typical of size 5-20 μm in diameter. The spectral detection part of the system consisted of a grating and a PMT array with 32 channels, which measured fluorescence in the wavelength from 280 nm to 800 nm. The detector system was used to demonstrate the measurement of laser induced fluorescence spectra of individual BWA simulant particles by excitation of single UV laser pulses. The spectrum obtained by averaging spectra from several BWA aerosol simulant particles were found generally similar, but not identical, to the fluorescence spectrum obtained from water solutions containing the same particles dissolved.

Paper Details

Date Published: 28 October 2005
PDF: 15 pages
Proc. SPIE 5990, Optically Based Materials and Optically Based Biological and Chemical Sensing for Defence II, 59900M (28 October 2005); doi: 10.1117/12.630141
Show Author Affiliations
Per Jonsson, Swedish Defence Research Agency (Sweden)
Fredrik Kullander, Swedish Defence Research Agency (Sweden)
Pär Wästerby, Swedish Defence Research Agency (Sweden)
Mikael Tiihonen, Royal Institute of Technology (Sweden)
Mikael Lindgren, Swedish Defence Research Agency (Sweden)
Norwegian Univ. of Science and Technology (Norway)

Published in SPIE Proceedings Vol. 5990:
Optically Based Materials and Optically Based Biological and Chemical Sensing for Defence II
Anthony W. Vere; John C. Carrano; Arturas Zukauskas; James G. Grote; Francois Kajzar, Editor(s)

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