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Proceedings Paper

Towards an integrated coherent anti-Stokes Raman scattering (CARS) microscopy system
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Paper Abstract

CARS microscopy has emerged as a powerful tool in imaging of biological matter. In addition to a high-3D spatial resolution, the technique delivers an attractive set of properties such as chemical specificity, high sensitivity, and fast data acquisition rates thus making it very suitable for biomedical applications. However, these advantages come at a cost of complex tunable laser sources, beam guiding and delivery optics. In particular, two high intensity laser pulses, whose carrier frequencies ωp (pump) and ωs (Stokes) are separated by the corresponding Raman shift value, are required to interact with the imaged media. In this paper, we present experimental results corresponding to our first step towards an integrated CARS-microscope. We demonstrate optical fiber delivery of two color picosecond pulses before they interact in the focus of microscope objective in order to produce CARS image. Certain aspects related to the effect of the pump and Stokes pulse parameters on image quality (e.g. contrast, sensitivity) after the pulses' propagation in the fiber will be addressed. The incorporation of fiber delivery feature significantly improves the microscope performance and ease its operation. In addition, we are exploring certain approaches in further development of CARS-microscope as a biomedical tool towards fully functional endoscope for in vivo chemically sensitive imaging.

Paper Details

Date Published: 30 September 2005
PDF: 9 pages
Proc. SPIE 5971, Photonic Applications in Nonlinear Optics, Nanophotonics, and Microwave Photonics, 597108 (30 September 2005); doi: 10.1117/12.629926
Show Author Affiliations
Francois Legare, Harvard Univ. (United States)
Feruz Ganikhanov, Harvard Univ. (United States)
Wellman Ctr. for Photomedicine, MGH, Harvard Medical School (United States)
X. Sunney Xie, Harvard Univ. (United States)

Published in SPIE Proceedings Vol. 5971:
Photonic Applications in Nonlinear Optics, Nanophotonics, and Microwave Photonics
Roberto A. Morandotti; Harry E. Ruda; Jianping Yao, Editor(s)

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