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Proceedings Paper

Immobilized gold nanoparticles fabricated by template assisted organo metallic chemical vapor deposition for oligonucleotide hybridisaton studies
Author(s): Anne Kathrena A. Aliganga; Fei Xu; Wolfgang Knoll; Silvia Mittler
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Paper Abstract

Immobilized gold nanoparticles on oxide surfaces were prepared by template assisted organometallic chemical vapor deposition (OMCVD). The growth parameters, optical parameters such as the plasmon resonance band, as well as geometrical parameters such as size and shape are investigated by UV-Vis spectroscopy, AFM, TEM and SEM. These new kind of gold nanoparticles were applied on a fluorescence based oligonucleotide hybridization study. Gold nanoparticles with a mean lateral diameter of 12 nm yielding an extinction maximum at around 520-530 nm should be able to resonantly excite the Cy3-fluorescence label attached to the target oligonucleotide. The hybridization reaction is taking place between gold nanoparticle surface-attached oligonucleotide catcher strands and chromophore-labeled target strands in solution. The experiment was conducted in the form of classical total internal reflection fluorescence detection. The kinetic data were quantitatively analyzed following a simple Langmuir model. It was found that a single mismatch between oligonucleotide target and probe reduces the Langmuir equilibrium constant by two orders of magnitude, allowing for an excellent sequence-specific detection of oligonucleotide hybridization based on OMCVD gold nanoparticles.

Paper Details

Date Published: 12 October 2005
PDF: 12 pages
Proc. SPIE 5969, Photonic Applications in Biosensing and Imaging, 59690H (12 October 2005); doi: 10.1117/12.629158
Show Author Affiliations
Anne Kathrena A. Aliganga, Max-Planck Institute for Polymer Research (Germany)
The Univ. of Western Ontario (Canada)
Fei Xu, Max-Planck Institute for Polymer Research (Germany)
Wolfgang Knoll, Max-Planck Institute for Polymer Research (Germany)
Silvia Mittler, The Univ. of Western Ontario (Canada)

Published in SPIE Proceedings Vol. 5969:
Photonic Applications in Biosensing and Imaging
Brian C. Wilson; Richard I. Hornsey; Warren C. W. Chan; Ulrich J. Krull; Robert A. Weersink; Kui Yu, Editor(s)

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