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Proceedings Paper

In-vivo corneal nonlinear optical tomography based on second harmonic and multiphoton autofluorescence imaging induced by near-infrared femtosecond lasers with rabbits
Author(s): Baogui Wang; Karl-Juergen Halbhuber; Iris Riemann; Karsten Koenig
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Paper Abstract

The intratissue multiphoton autofluorescence imaging (MAI) and the second harmonic generation (SHG) based on nonlinear process of femtosecond nanojoule laser pulses at wave length of 750-850 nm emitted from solid-state Titanium: Sapphire Chameleon have been used as a highly precise non-destructive tool to realize the in-vivo differentiation of corneal layers with the assistance of intratissue optical tomography and to visualize the keratocyte structures and collagen lamellas with submicron resolution. Multiphoton nonlinear imaging occurs only with high light intensity on an order of MG-GW/cm2 and photon flux density of more than 1024 photons cm-2s-1 in a 0.1femtoliter intrastromal focus volume obtained by diffraction-limited focussing with high-numerical objectives. This technique, acting as a novel diagnostic tool, proved to be essential for femtosecond (fs) nanojoule (nJ) cornea surgery to determine the interest of region preoperation, to visualize and verify the outcomes immediately after the laser surgery and has potential to become a powerful tool in advancing understanding of corneal biomechnics and cellular reactions after laser induced lesion.

Paper Details

Date Published: 14 October 2005
PDF: 11 pages
Proc. SPIE 5964, Detectors and Associated Signal Processing II, 59640O (14 October 2005); doi: 10.1117/12.622204
Show Author Affiliations
Baogui Wang, Univ. of Jena (Germany)
Karl-Juergen Halbhuber, Univ. of Jena (Germany)
Iris Riemann, Fraunhofer Institute of Biomedical Technology (Germany)
Karsten Koenig, Fraunhofer Institute of Biomedical Technology (Germany)
Univ. of Saarland (Germany)


Published in SPIE Proceedings Vol. 5964:
Detectors and Associated Signal Processing II
Jean-Pierre Chatard; Peter N. J. Dennis, Editor(s)

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