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Proceedings Paper

Molecular binding and enhanced photoluminescence of bromocresol purple in marine derived DNA
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Paper Abstract

Deoxyribonucleic acid (DNA) extracted and purified from salmon roe and milt sacs, a waste product of the fishing industry was studied for molecular binding and photoluminescence effects using bromocresol purple (BCP). Since BCP is both water and alcohol soluble it was investigated for binding efficiency in DNA/water solutions and modified DNA-CTMA/butanol solutions. Circular dichroism studies show that there is a maximum binding concentration of BCP in the DNA/water solution at ~5% by weight of BCP:DNA. In contrast, DNA-CTMA/butanol solutions showed increased binding concentrations up through 10wt% BCP:DNA-CTMA. This apparent binding affinity of DNA-CTMA for BCP also resulted in a significantly higher (6x) photoluminescence in thin film form when compared to BCP:PMMA films of the same doping concentration.

Paper Details

Date Published: 18 August 2005
PDF: 8 pages
Proc. SPIE 5934, Nonlinear Optical Transmission and Multiphoton Processes in Organics III, 593407 (18 August 2005); doi: 10.1117/12.621954
Show Author Affiliations
Darnell E. Diggs, Air Force Research Lab. (United States)
Josh Hagen, Univ. of Cincinnati (United States)
Zhou Yu, Univ. of Cincinnati (United States)
Emily Heckman, Univ. of Dayton (United States)
Anteon Corp. (United States)
F. Kenneth Hopkins, Air Force Research Lab. (United States)
James G. Grote, Air Force Research Lab. (United States)
Andrew Steckl, Univ. of Cincinnati (United States)

Published in SPIE Proceedings Vol. 5934:
Nonlinear Optical Transmission and Multiphoton Processes in Organics III
A. Todd Yeates, Editor(s)

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