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Proceedings Paper

Effect of scanning rate on the image contrast in confocal microscopy for biological application
Author(s): Byung Seon Chun; Tae Joong Kim; Incheon Song; Dae-Gab Gweon; Jaebum Choo; Chil Hwan Oh
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Paper Abstract

In this research, the method how to estimate the image quality for different scanning rate is suggested and experimentally shown with the laboratory-built confocal laser scanning microscope. The confocal microscope is designed for in vivo reflectance imaging of a biological tissue, which uses the refractive index mismatch at the boundaries of a tissue to generate an image without any additional staining process. The two-dimensional scanning mechanism is built up with a polygonal mirror and a galvanometric mirror that can be controlled to operate at a specific speed. To examine the effect of scanning rate on the image contrast, confocal scanning images of a biological specimen are acquired with various scanning rate while the other conditions are kept same. The contrast of confocal microscopic image is transformed into the numeric expression to describe the relation between image contrast and scanning rate quantitatively. Results suggest some useful methodology of how to determine the allowable maximum scanning rate for the specific application of confocal microscopy.

Paper Details

Date Published: 1 August 2005
PDF: 8 pages
Proc. SPIE 5873, Optical Scanning 2005, (1 August 2005); doi: 10.1117/12.615825
Show Author Affiliations
Byung Seon Chun, KAIST (South Korea)
Tae Joong Kim, KAIST (South Korea)
Incheon Song, KAIST (South Korea)
Dae-Gab Gweon, KAIST (South Korea)
Jaebum Choo, Hanyang Univ. (South Korea)
Chil Hwan Oh, Korea Univ. Medical Ctr. (South Korea)

Published in SPIE Proceedings Vol. 5873:
Optical Scanning 2005
Stephen F. Sagan; Gerald F. Marshall, Editor(s)

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