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Proceedings Paper

Influence of beam shape on in-vitro cellular transformations in human skin fibroblasts
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Paper Abstract

A variety of strategies have been utilised for prevention and treatment of chronic wounds such as leg ulcers, diabetic foot ulcers and pressure sores1. Low Level Laser Therapy (LLLT) has been reported to be an invaluable tool in the enhancement of wound healing through stimulating cell proliferation, accelerating collagen synthesis and increasing ATP synthesis in mitochondria to name but a few2. This study focused on an in-vitro analysis of the cellular responses induced by treatment with three different laser beam profiles namely, the Gaussian (G), Super Gaussian (SG) and Truncated Gaussian (TG), on normal wounded irradiated (WI) and wounded non-irradiated (WNI) human skin fibroblast cells (WS1), to test their influence in wound healing at 632.8 nm using a helium neon (HeNe) laser. For each beam profile, measurements were made using average energy densities over the sample ranging from 0.2 to 1 J, with single exposures on normal wounded cells. The cells were subjected to different post irradiation incubation periods, ranging from 0 to 24 hours to evaluate the duration (time) dependent effects resulting from laser irradiation. The promoted cellular alterations were measured by increase in cell viability, cell proliferation and cytotoxicity. The results obtained showed that treatment with the G compared to the SG and TG beams resulted in a marked increase in cell viability and proliferation. The data also showed that when cells undergo laser irradiation some cellular processes are driven by the peak energy density rather than the energy of the laser beam. We show that there exist threshold values for damage, and suggest optimal operating regimes for laser based wound healing.

Paper Details

Date Published: 30 August 2005
PDF: 10 pages
Proc. SPIE 5876, Laser Beam Shaping VI, 58760V (30 August 2005); doi: 10.1117/12.614950
Show Author Affiliations
Patience Mthunzi, CSIR (South Africa)
Andrew Forbes, CSIR (South Africa)
Denise Hawkins, University of Johannesburg (South Africa)
Heidi Abrahamse, University of Johannesburg (South Africa)
Aletta E. Karsten, CSIR (South Africa)

Published in SPIE Proceedings Vol. 5876:
Laser Beam Shaping VI
Fred M. Dickey; David L. Shealy, Editor(s)

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