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Proceedings Paper

Measuring fungal growth forces with optical tweezers
Author(s): Graham D. Wright; Jochen Arlt; Wilson C. K. Poon; Nick D. Read
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Paper Abstract

Optical tweezers are proving themselves useful and powerful tools in many areas of biological research, including fungal cell biology. Here we investigate their potential as a novel method for measuring the growth forces of fungal hyphae. We have calibrated the trapping forces produced by our tweezers system, and using beads have investigated whether growing hyphae can overcome this force and push beads out of the trap. Although 4 μm beads were pushed out of the trap by fungal hyphae, using 1 μm beads produced some more intriguing results. The problems encountered with this method of measurement and possible solutions are discussed.

Paper Details

Date Published: 18 August 2005
PDF: 7 pages
Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 59300F (18 August 2005); doi: 10.1117/12.614512
Show Author Affiliations
Graham D. Wright, Univ. of Edinburgh (United Kingdom)
Jochen Arlt, Univ. of Edinburgh (United Kingdom)
Wilson C. K. Poon, Univ. of Edinburgh (United Kingdom)
Nick D. Read, Univ. of Edinburgh (United Kingdom)

Published in SPIE Proceedings Vol. 5930:
Optical Trapping and Optical Micromanipulation II
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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