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Proceedings Paper

Stretched exponential decay and correlations from two-state single molecule trajectories
Author(s): Ophir Flomenbom; Joseph Klafter
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Paper Abstract

Single molecule techniques offer a unique tool studying the dynamical behaviour of individual molecules, and provide the possibility to construct distributions from individual events rather than from a signal stemming from an ensemble of molecules. Observing the activity of individual lipase molecules for extended periods of time (hours), we get long trajectories, made of "on-state" and "off-state" events. The waiting time probability density function (PDF) of the off-state and the state-correlaiton function fit stretched exponentials, independent of the substrate concentration in a certain range. The data analysis unravels correlations between off-state events. These findings imply that the fluctuating enzyme model, which involves a spectrum of enzymatic conformations that interconvert on the timescale of the catalytic activity, best describes the observed enzymatic activity. This contribution summarizes some of our recent published results.

Paper Details

Date Published: 23 May 2005
PDF: 8 pages
Proc. SPIE 5845, Noise in Complex Systems and Stochastic Dynamics III, (23 May 2005); doi: 10.1117/12.610133
Show Author Affiliations
Ophir Flomenbom, Tel Aviv Univ. (Israel)
Joseph Klafter, Tel Aviv Univ. (Israel)

Published in SPIE Proceedings Vol. 5845:
Noise in Complex Systems and Stochastic Dynamics III
Laszlo B. Kish; Katja Lindenberg; Zoltan Gingl, Editor(s)

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