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Proceedings Paper

Novel polymer platform for enhanced biochip performance
Author(s): Helen M. McEvoy; Robert Blue; Nigel Kent; Lubos Polerecky; Colette McDonagh; Brian D. MacCraith
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Paper Abstract

We report the development of enhanced optical platforms for fluorescence-based biosensors. A previous analysis by us has shown that the emission of fluorescence in such a system is highly anisotropic and is preferentially emitted into the substrate over a well-defined angular range, with the result that the light is guided along the substrate via total internal reflection. However, conventional optical biosensors based on fluorescence detection typically employ a detector that is positioned either directly above or directly below the biochip. As a consequence, only a small fraction of the total emitted fluorescence is detected, which impacts adversely on sensor performance. The enhanced biosensor presented here is based on a novel, generic platform specifically designed to overcome the inherent limitations of planar substrates. The platform incorporates custom-designed optical elements, the purpose of which is to redirect the emitted fluorescence onto a detector positioned beneath the biochip. Platforms were fabricated using the polymer processing technique of microinjection moulding. In this paper we demonstrate the ability of this optical system to achieve a 80-fold luminescence capture enhancement. We also demonstrate its effectiveness as an enhanced biosensor platform by carrying out a proof of principle BSA/antiBSA competitive assay. This work has significant implications for the development of mass-producible, highly efficient optical biosensors.

Paper Details

Date Published: 3 June 2005
PDF: 8 pages
Proc. SPIE 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy, (3 June 2005); doi: 10.1117/12.606423
Show Author Affiliations
Helen M. McEvoy, Dublin City Univ. (Ireland)
Robert Blue, Dublin City Univ. (Ireland)
Nigel Kent, Dublin City Univ. (Ireland)
Lubos Polerecky, Dublin City Univ. (Ireland)
Colette McDonagh, Dublin City Univ. (Ireland)
Brian D. MacCraith, Dublin City Univ. (Ireland)

Published in SPIE Proceedings Vol. 5826:
Opto-Ireland 2005: Optical Sensing and Spectroscopy
Gerard D. O'Sullivan; Brian D. MacCraith; Hugh James Byrne; Enda McGlynn; Alan G. Ryder, Editor(s)

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