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Proceedings Paper

Surface-enhanced Raman scattering molecular nanoprobes
Author(s): Musundi B. Wabuyele; Fei Yan; Guy D Griffin; Tuan Vo-Dinh
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Paper Abstract

Nanoparticles are increasingly finding a wide application in the biological studies due to their unique physical and chemical properties. Colloidal nanoparticles are efficient substrate that exhibit surface-enhanced Raman scattering (SERS) phenomenon by enhancing the scattering cross-sections of conjugated Raman active molecules thus enabling highly sensitive biological probes. However, biological and medical applications would require nanoparticles to be conjugated to biomolecules. A universal approach for conjugation of mercarptoacetic acid-capped silver nanoparticles to biomolecules is described. The surface functionalized silver colloids were labeled with a Raman active dye and used for cellular imaging. We also described the use of silver nanoparticle to develop a new class of SERS nanoprobes for molecular recognition and detection of specific nucleic acid sequences.

Paper Details

Date Published: 1 April 2005
PDF: 7 pages
Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); doi: 10.1117/12.604447
Show Author Affiliations
Musundi B. Wabuyele, Oak Ridge National Lab. (United States)
Fei Yan, Oak Ridge National Lab. (United States)
Guy D Griffin, Oak Ridge National Lab. (United States)
Tuan Vo-Dinh, Oak Ridge National Lab. (United States)

Published in SPIE Proceedings Vol. 5692:
Advanced Biomedical and Clinical Diagnostic Systems III
Tuan Vo-Dinh; Warren S. Grundfest M.D.; David A. Benaron M.D.; Gerald E. Cohn, Editor(s)

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