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Proceedings Paper

The fluorescence intensity of the quantum dots in the water depends on the surface processing
Author(s): Kenji Yamamoto
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Paper Abstract

The longer the excitation light is dosed on the quantum dots, the larger the intensity of the fluorescent light we get. It is known as the light memory effect. This effect was found in dry film of quantum dot at first. We found this memory effect also occurs inside the cell and also found the wavelength of the fluorescent light to shift toward shorter. In order to acquire the water solubility, we use mercapto undecanic acids, mercapto glycerol, and mercapto amine for the surface treatment. The base line of the fluorescent intensity is the highest with the quantum dot treated with mercapto undecanoic acids. That with the mercapto glycerol is the next. That with the mercapto amine is the lowest among the three compounds. We measured the zeta potential of the surface of these three different treated quantum dot. The quantum dot treated with the mercapto undecanoic acids is negatively charged, that with the mercapto amine is positively charged and that with the mercapto glycerol is the neutral. We added the electron donor components and the electron scavenger components and found that the electron donor components raises up the intensity of the fluorescent light and the electron scavenger components puts down the intensity, as expected. The conjugation of the quantum dot with the bio-molecule such as protein, sugar and nucleic acids will change the zeta potential which lead to speculate that the measurement of the zeta potential of the bio-molecule could predict roughly the fluorescent intensity of the conjugated bio-molecule and quantum dot complex.

Paper Details

Date Published: 28 April 2005
PDF: 7 pages
Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.601513
Show Author Affiliations
Kenji Yamamoto, Research Institute of the International Medical Ctr. of Japan (Japan)

Published in SPIE Proceedings Vol. 5705:
Nanobiophotonics and Biomedical Applications II
Alexander N. Cartwright; Marek Osinski, Editor(s)

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