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Proceedings Paper

Long-term in vivo harmonics imaging of zebrafish embryonic development based on a femtosecond Cr:forsterite laser
Author(s): S.-Y. Chen; T.-H. Tsai; C.-S. Hsieh; S.-P. Tai; C.-Y. Lin; C.-Y. Ko; Y.-C. Chen; H.-J. Tsai; C.-H. Hu; C.-K. Sun
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Paper Abstract

Based on a femtosecond Cr:forsterite laser, harmonics optical microscopy (HOM) provides a truly “noninvasive” tool for in vivo and long-term study of vertebrate embryonic development. Based on optical nonlinearity, HOM provides sub-micrometer 3D spatial resolution and high 3D optical-sectioning power without using invasive and toxic fluorophores. Since only virtual-level-transition is involved, HOM is known to leave no energy deposition and no photodamage. Combined with second harmonic generation, which is sensitive to specific structure such as nerve and muscle fibers, HOM can perform functional studies of early developmental dynamics of many vertebrate physiological systems. Recently, zebrafish has become a standard model for many biological and medical studies of vertebrates, due to the similarity between embryonic development of zebrafish and human being. Here we demonstrate in vivo HOM studies of developmental dynamics of several important embryonic physiological systems in live zebrafish embryos, with focuses on the developments of brains, eyes, ears, and hearts. Based on a femtosecond Cr:forsterite laser, which provides the deepest penetration (~1.5mm) and least photodamage in the zebrafish embryo, complete developing processes of different physiological systems within a period of time longer than 20 hours can be non-invasively observed inside the same embryo.

Paper Details

Date Published: 21 March 2005
PDF: 8 pages
Proc. SPIE 5714, Commercial and Biomedical Applications of Ultrafast Lasers V, (21 March 2005); doi: 10.1117/12.600829
Show Author Affiliations
S.-Y. Chen, National Taiwan Univ. (Taiwan)
T.-H. Tsai, National Taiwan Univ. (Taiwan)
C.-S. Hsieh, National Taiwan Univ. (Taiwan)
S.-P. Tai, National Taiwan Univ. (Taiwan)
C.-Y. Lin, National Taiwan Univ. (Taiwan)
C.-Y. Ko, National Taiwan Ocean Univ. (Taiwan)
Y.-C. Chen, National Taiwan Ocean Univ. (Taiwan)
H.-J. Tsai, National Taiwan Univ. (Taiwan)
C.-H. Hu, National Taiwan Ocean Univ. (Taiwan)
C.-K. Sun, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 5714:
Commercial and Biomedical Applications of Ultrafast Lasers V
Joseph Neev; Christopher B. Schaffer; Andreas Ostendorf; Stefan Nolte, Editor(s)

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