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Proceedings Paper

Improved 3D cellular imaging by multispectral focus assessment
Author(s): Tong Zhao; Yizhi Xiong; Alice P. Chung; Elliot S. Wachman; Daniel L. Farkas
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Paper Abstract

Biological specimens are three-dimensional structures. However, when capturing their images through a microscope, there is only one plane in the field of view that is in focus, and out-of-focus portions of the specimen affect image quality in the in-focus plane. It is well-established that the microscope’s point spread function (PSF) can be used for blur quantitation, for the restoration of real images. However, this is an ill-posed problem, with no unique solution and with high computational complexity. In this work, instead of estimating and using the PSF, we studied focus quantitation in multi-spectral image sets. A gradient map we designed was used to evaluate the sharpness degree of each pixel, in order to identify blurred areas not to be considered. Experiments with realistic multi-spectral Pap smear images showed that measurement of their sharp gradients can provide depth information roughly comparable to human perception (through a microscope), while avoiding PSF estimation. Spectrum and morphometrics-based statistical analysis for abnormal cell detection can then be implemented in an image database where the axial structure has been refined.

Paper Details

Date Published: 29 March 2005
PDF: 11 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.597586
Show Author Affiliations
Tong Zhao, Cedars-Sinai Medical Ctr. (United States)
Carnegie Mellon Univ. (United States)
Yizhi Xiong, Cedars-Sinai Medical Ctr. (United States)
Alice P. Chung, Cedars-Sinai Medical Ctr. (United States)
Elliot S. Wachman, ChromoDynamics, Inc. (United States)
Daniel L. Farkas, Cedars-Sinai Medical Center (United States)
Carnegie Mellon Univ. (United States)

Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

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