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Proceedings Paper

Development of an SH-SAW sensor for detection of DNA immobilization and hybridization
Author(s): Yongrae Roh; Jeongdong Woo; Youngjune Hur; Yukeun Eugene Pak
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Paper Abstract

We have developed SH (shear horizontal) surface acoustic wave (SAW) sensors for detection of the immobilization and hybrdization of DNA (deoxyribonucleic acid) on the gold coated delay line of transverse SAW devices. The experiments of DNA immobilization and hybridization were performed with 15-mer oligonucleotides (probe and complementary target DNA). The sensor consists of twin SAW delay line oscillators (sensing channel and reference channel) operating at 100 MHz fabricated on 36° rotated Y-cut X-propagation LiTaO3 piezoelectric single crystals. The relative change in the frequency of the two oscillators was monitored to detect the immobilization of probe DNA with thiol group on the Au coated delay line and the hybridization between target DNA and immobilized probe DNA in a pH 7.4 PBS (phosphate buffered saline) solution. The measurement results showed a good response of the sensor to the mass loading effects of the DNA immobilization and hybridization with the sensitivity up to 1.5 ng/ml/Hz.

Paper Details

Date Published: 16 May 2005
PDF: 10 pages
Proc. SPIE 5763, Smart Structures and Materials 2005: Smart Electronics, MEMS, BioMEMS, and Nanotechnology, (16 May 2005); doi: 10.1117/12.597399
Show Author Affiliations
Yongrae Roh, Kyungpook National Univ. (South Korea)
Jeongdong Woo, Kyungpook National Univ. (South Korea)
Youngjune Hur, Kyungpook National Univ. (South Korea)
Yukeun Eugene Pak, Samsung Advanced Institute of Technolgy (South Korea)

Published in SPIE Proceedings Vol. 5763:
Smart Structures and Materials 2005: Smart Electronics, MEMS, BioMEMS, and Nanotechnology
Vijay K. Varadan, Editor(s)

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