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Proceedings Paper

Visualizing 3D microscopic specimens
Author(s): Per-Ola Forsgren; Lars L. Majlof
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Paper Abstract

The confocal microscope can be used in a vast number of fields and applications to gather more information than is possible with a regular light microscope, in particular about depth. Compared to other three-dimensional imaging devices such as CAT, NMR, and PET, the variations of the objects studied are larger and not known from macroscopic dissections. It is therefore important to have several complementary ways of displaying the gathered information. We present a system where the user can choose display techniques such as extended focus, depth coding, solid surface modeling, maximum intensity and other techniques, some of which may be combined. A graphical user interface provides easy and direct control of all input parameters. Motion and stereo are available options. Many three- dimensional imaging devices give recordings where one dimension has different resolution and sampling than the other two which requires interpolation to obtain correct geometry. We have evaluated algorithms with interpolation in object space and in projection space. There are many ways to simplify the geometrical transformations to gain performance. We present results of some ways to simplify the calculations.

Paper Details

Date Published: 26 June 1992
PDF: 7 pages
Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); doi: 10.1117/12.59607
Show Author Affiliations
Per-Ola Forsgren, Molecular Dynamics (United States)
Lars L. Majlof, Molecular Dynamics (United States)

Published in SPIE Proceedings Vol. 1660:
Biomedical Image Processing and Three-Dimensional Microscopy
Raj S. Acharya; Carol J. Cogswell; Dmitry B. Goldgof, Editor(s)

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