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Proceedings Paper

High-resolution confocal transmission microscope, Part II: determining image position and correcting aberrations
Author(s): John W. O'Byrne; Carol J. Cogswell
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Paper Abstract

In order to produce high resolution images of complex biological specimens in a confocal transmission microscope, two phenomena must be overcome. Firstly, non-uniform refractive index variations in the specimen cause deflection of the focused image spot in three- dimensional space which presents a problem for the usual confocal approach of imaging onto a fixed pinhole. The second effect is optical aberrations (especially spherical aberration) which arise as a natural function of imaging through the thickness of a refractive specimen. This paper discusses how both effects may be monitored and overcome, while still providing confocal imaging, using a CCD array as the detector

Paper Details

Date Published: 26 June 1992
PDF: 9 pages
Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); doi: 10.1117/12.59580
Show Author Affiliations
John W. O'Byrne, Univ. of Sydney (Australia)
Carol J. Cogswell, Univ. of Sydney (Australia)

Published in SPIE Proceedings Vol. 1660:
Biomedical Image Processing and Three-Dimensional Microscopy
Raj S. Acharya; Carol J. Cogswell; Dmitry B. Goldgof, Editor(s)

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