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Proceedings Paper

Fluorescence photometric analysis of Photofrin uptake and the detection of precancerous and true epidermoid lesions
Author(s): Thomas S. Mang; David H. Crean; Frank T. Sindoni; Charles Liebow
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Paper Abstract

Neoplastic tissue can be detected by its increased fluorescence compared to surrounding normal tissue after the injection of the tumor-localizing compound Photofrin®. In vivo fluorescence photometry is a non-imaging photodetector which detects the 690 nm fluorescence of the porphyrin. The sensitivity of the instrumentation has allowed the detection of micrometastases in both pre-clinical and clinical studies using low, non-photosensitizing levels of the drug. The technique is now being applied to the 9,10 dimethyl-1,2-.benzanthracene (DMBA)induced hamster buccal cheek pouch carcinoma model to obtain data on the correlation between Photofrmn® uptake and tumor development. This model shows consistent time patterns of tumor development as well precancerous leukoplakia lesions and has been well documented as an animal model of oral epidermoid carcinogenesis. The buccal cheek pouches of Syrian Golden hamsters were exposed to a 0.5% DMBA in acetone thrice weekly for specified time durations. Hamsters were subsequently injected with 1.0 mgfkg of Photofrmn® within the various stages of tumor development. Twenty-four hours post-injection, fluorescence due to drug uptake was measured by in vivo fluorescence photometry. Mucosal tissues were subsequently biopsied and used for extraction assays. Results demonstrate that Photoflin® is retained in DMBA treated tissue with a linear relationship between length of application and Photofrin® uptake and fluorescence. This relationship establishes that premalignant lesions can be distinguished from normal tissue by Photofrmn® uptake and fluorescence and suggest that Photofrmn® uptake and fluorescence can be used in a predictive manner to diagnose and determine the progression of individual lesions.

Paper Details

Date Published: 6 May 1992
PDF: 9 pages
Proc. SPIE 1641, Physiological Monitoring and Early Detection Diagnostic Methods, (6 May 1992); doi: 10.1117/12.59352
Show Author Affiliations
Thomas S. Mang, Roswell Park Cancer Institute (United States)
David H. Crean, Roswell Park Cancer Institute (United States)
Frank T. Sindoni, SUNY/Buffalo School of Dental Medicine (United States)
Charles Liebow, SUNY/Buffalo School of Dental Medicine (United States)

Published in SPIE Proceedings Vol. 1641:
Physiological Monitoring and Early Detection Diagnostic Methods
Thomas S. Mang, Editor(s)

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