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Proceedings Paper

pH microprobe manipulated in microchannels using optical tweezers
Author(s): Gavin S. Sinclair; Norbert Klauke; Paul Monaghan; Miles J. Padgett; Jon Cooper
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Paper Abstract

SNARF-1 fluorochrome was used to functionalize 3μm diameter latex spheres making them sensitive to the pH of their environment, manifested as a change in their fluorescence. The fluorescence emission at 580nm was excited using a filtered xenon arc lamp at 515nm. A solution of functionalized latex spheres was placed between gold microelectrodes in a microfluidic channel. Optical tweezers were used to trap and manipulate the spheres in the vicinity of the microelectrodes, to map out the pH profile in the electrolyte solution, induced by passing 20 microsecond transient current pulses through the microelectrodes.

Paper Details

Date Published: 14 March 2005
PDF: 7 pages
Proc. SPIE 5736, Nanomanipulation with Light, (14 March 2005); doi: 10.1117/12.593307
Show Author Affiliations
Gavin S. Sinclair, Univ. of Glasgow (United Kingdom)
Norbert Klauke, Univ. of Glasgow (United Kingdom)
Paul Monaghan, Univ. of Glasgow (United Kingdom)
Miles J. Padgett, Univ. of Glasgow (United Kingdom)
Jon Cooper, Univ. of Glasgow (United Kingdom)


Published in SPIE Proceedings Vol. 5736:
Nanomanipulation with Light
David L. Andrews, Editor(s)

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