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Proceedings Paper

A common-path phase-shift interferometry surface plasmon imaging system
Author(s): Y.-T. Su; Shean-Jen Chen; T.-L. Yeh
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Paper Abstract

A biosensing imaging system is proposed based on the integration of surface plasmon resonance (SPR) and common-path phase-shift interferometry (PSI) techniques to measure the two-dimensional spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR phase imaging system can offer high resolution and high-throughout screening capabilities to analyze microarray biomolecular interaction without the need for additional labeling. With the long-term stability advantage of the common-path PSI technique even with external disturbances such as mechanical vibration, buffer flow noise, and laser unstable issue, the system can match the demand of real-time kinetic study for biomolecular interaction analysis (BIA). The SPR-PSI imaging system has achieved a detection limit of 2×10-7 refraction index change, a long-term phase stability of 2.5x10-4π rms over four hours, and a spatial phase resolution of 10-3 π with a lateral resolution of 100μm.

Paper Details

Date Published: 31 March 2005
PDF: 8 pages
Proc. SPIE 5703, Plasmonics in Biology and Medicine II, (31 March 2005);
Show Author Affiliations
Y.-T. Su, National Cheng Kung Univ. (Taiwan)
Shean-Jen Chen, National Cheng Kung Univ. (Taiwan)
T.-L. Yeh, National Central Univ. (Taiwan)

Published in SPIE Proceedings Vol. 5703:
Plasmonics in Biology and Medicine II
Tuan Vo-Dinh; Joseph R. Lakowicz; Zygmunt K. Gryczynski, Editor(s)

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