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Proceedings Paper

Measuring the effects of topically applied skin optical clearing agents and modeling the effects and consequences for laser therapies
Author(s): Wim Verkruysse; Misbah Khan M.D.; Bernard Choi; Lars O. Svaasand; J. Stuart Nelson M.D.
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Paper Abstract

Human skin prepared with an optical clearing agent manifests reduced scattering as a result of de-hydration and refractive index matching. This has potentially large effects for laser therapies of several skin lesions such as port wine stain, hair removal and tattoo removal. With most topically applied clearing agents the clearing effect is limited because they penetrate poorly through the intact superficial skin layer (stratum corneum). Agent application modi other than topical are impractical and have limited the success of optical clearing in laser dermatology. In recent reports, however, a mixture of lipofylic and hydrofylic agents was shown to successfully penetrate through the intact stratum corneum layer which has raised new interest in this field. Immediately after application, the optical clearing effect is superficial and, as the agent diffuses through the skin, reduced scattering is manifested in deeper skin layers. For practical purposes as well as to maximize therapeutic success, it is important to quantify the reduced scattering as well as the trans-cutaneous transport dynamics of the agent. We determined the time and tissue depth resolved effects of optically cleared skin by inserting a microscopic reflector array in the skin. Depth dependent light intensity was measured by quantifying the signal of the reflector array with optical coherence tomography. A 1-dimensional mass diffusion model was used to estimate a trans-cutaneous transport diffusion constant for the clearing agent mixture. The results are used in Monte Carlo modeling to determine the optimal time of laser treatment after topical application of the optical clearing agent.

Paper Details

Date Published: 15 April 2005
PDF: 10 pages
Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); doi: 10.1117/12.591421
Show Author Affiliations
Wim Verkruysse, Univ. of California/Irvine (United States)
Misbah Khan M.D., Univ. of California/Irvine (United States)
Bernard Choi, Univ. of California/Irvine (United States)
Lars O. Svaasand, Norwegian Univ. of Science and Technology (Norway)
J. Stuart Nelson M.D., Univ. of California/Irvine (United States)

Published in SPIE Proceedings Vol. 5695:
Optical Interactions with Tissue and Cells XVI
Steven L. Jacques; William P. Roach, Editor(s)

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