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Proceedings Paper

A fluorescence microscopy study of quantum dots as fluorescent probes for brain tumor diagnosis
Author(s): Jingjing Wang; P. Thomas Vernier; Yinghua Sun; Martin A. Gundersen; Laura Marcu
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Paper Abstract

In vivo fluorescent spectroscopy and imaging using endogenous and exogenous sources of contrast can provide new approaches for enhanced demarcation of brain tumor margins and infiltration. Quantum dots (QDs), nanometer-size fluorescent probes, represent excellent contrast agents for biomedical imaging due to their broader excitation spectrum, narrower emission spectra, and higher sensitivity and stability. The epidermal growth factor receptor (EGFR) is implicated in the development and progression of a number of human solid tumors including brain tumors and thus a potential target for brain tumor diagnosis. In this study, we investigate the up-take of ODs by brain tumor cells and the potential use of EGFR-targeted QDs for enhanced optical imaging of brain tumors. We conducted fluorescence microscopy studies of the up-take mechanism of the anti-EGFR-ODs complexes by Human U87, and SKMG-3 glioblastoma cells. Our preliminary results show that QDs can enter into glioma cells through anti-EGFR mediated endocytosis, suggesting that these nano-size particles can tag brain tumor cells.

Paper Details

Date Published: 31 March 2005
PDF: 8 pages
Proc. SPIE 5703, Plasmonics in Biology and Medicine II, (31 March 2005); doi: 10.1117/12.591385
Show Author Affiliations
Jingjing Wang, Univ. of Southern California (United States)
P. Thomas Vernier, MOSIS, Information Sciences Institute (United States)
Yinghua Sun, Univ. of Southern California (United States)
Martin A. Gundersen, Univ. of Southern California (United States)
Laura Marcu, Univ. of Southern California (United States)
Cedars-Sinai Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 5703:
Plasmonics in Biology and Medicine II
Tuan Vo-Dinh; Joseph R. Lakowicz; Zygmunt K. Gryczynski, Editor(s)

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