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Proceedings Paper

Combined multiphoton and optical coherence microscopy
Author(s): Shuo Tang; Tatiana B. Krasieva; Zhongping Chen; Bruce J. Tromberg
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Paper Abstract

Multiphoton microscopy (MPM) and optical coherence tomography (OCT) are two important techniques for non-invasive and high-resolution imaging of highly scattering biomedical tissues. MPM and OCT provide complementary information about tissues as the detected signals originate from different substances and mechanisms. Combining MPM and OCT can simultaneously provide rich functionality and morphology information about biomedical tissues. We report a novel system which combines multiphoton and optical coherence microscopy. A femtosecond Ti:Sapphire laser is used as the light source which has a pulse width of 10 fs and a spectral bandwidth of 100 nm. The ultrafast Ti:Sapphire laser provides the short pulse and the broad bandwidth required by high-resolution MPM and OCT, respectively. By matching the resolution and the imaging volume of MPM and OCT, we achieved co-registration between the MPM and OCT images. Dispersion compensation is critical in this MPM/OCT system and its effect is discussed. Representative images of co-registered MPM/OCT are presented.

Paper Details

Date Published: 30 March 2005
PDF: 8 pages
Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); doi: 10.1117/12.591257
Show Author Affiliations
Shuo Tang, Beckman Laser Institute (United States)
Tatiana B. Krasieva, Beckman Laser Institute (United States)
Zhongping Chen, Beckman Laser Institute (United States)
Bruce J. Tromberg, Beckman Laser Institute (United States)

Published in SPIE Proceedings Vol. 5700:
Multiphoton Microscopy in the Biomedical Sciences V
Ammasi Periasamy; Peter T. C. So, Editor(s)

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