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Proceedings Paper

Data analysis considerations in probing single quantum dot fluorescence intermittency
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Paper Abstract

The fluorescence intermittency of single, bare, CdSe/ZnS quantum dots was probed using single molecule confocal microscopy and found to demonstrate power law kinetics. Various threshold values and line fitting parameters are employed in the data analysis and their effects on the extracted power law exponents, moff and mon, are presented. The threshold is found to be critical in determining moff while having no significant effect on mon. The mean plus 2σ threshold, calculated from the background noise in the measurement, results in a more negative moff slope in comparison to the mean plus 3σ or mean plus 4σ thresholds. This is likely due to the mean plus 2σ threshold lying within the background noise outliers which mimic short on events. In contrast, the mean plus 4σ threshold is above 99.99% of the background noise while adequately below the fluorescence signal. Additionally, it is found that fitting only the ten most probable data points rather than all the data points in the log-log probability density graphs results in no significant change in moff and mon.

Paper Details

Date Published: 28 April 2005
PDF: 8 pages
Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.590707
Show Author Affiliations
Jeffrey R. Krogmeier, National Institute of Standards and Technology (United States)
Jeeseong Hwang, National Institute of Standards and Technology (United States)

Published in SPIE Proceedings Vol. 5705:
Nanobiophotonics and Biomedical Applications II
Alexander N. Cartwright; Marek Osinski, Editor(s)

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