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Proceedings Paper

Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes
Author(s): Daniel R. Matthews; Huw D. Summers; Kerenza Njoh; Rachel J. Errington; Paul J. Smith; Simon Ameer-Beg; Boris Vojnovic
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Paper Abstract

We report on the development of a stroboscopic excitation technique using a self-pulsing laser, and show that it is a feasible method for obtaining fluorescence lifetime information from a biochip format. The self-pulsing lasers described here are versatile devices which have been used for one photon excitation measurements to determine the lifetime of cyanine 5 in water and ethanol. The same devices have been used to develop a technique whereby the emphasis for time-resolution of a lifetime measurement can be transferred to the excitation source from the detector and processing electronics by virtue of the multiple-pulse, variable frequency nature of the laser output.

Paper Details

Date Published: 28 April 2005
PDF: 9 pages
Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.590201
Show Author Affiliations
Daniel R. Matthews, Cardiff Univ. (United Kingdom)
Huw D. Summers, Cardiff Univ. (United Kingdom)
Kerenza Njoh, Cardiff Univ. (United Kingdom)
Rachel J. Errington, Cardiff Univ. (United Kingdom)
Paul J. Smith, Cardiff Univ. (United Kingdom)
Simon Ameer-Beg, Gray Cancer Institute (United Kingdom)
Boris Vojnovic, Gray Cancer Institute (United Kingdom)

Published in SPIE Proceedings Vol. 5705:
Nanobiophotonics and Biomedical Applications II
Alexander N. Cartwright; Marek Osinski, Editor(s)

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