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Proceedings Paper

Two-dimensional micro array fluorescence fingerprinting with a computer screen photo-assisted technique
Author(s): Jimmy W. P. Bakker; Daniel Filippini; Ingemar Lundstroem
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Paper Abstract

Detection and classification of fluorescent dyes are demonstrated using a computer screen photo-assisted technique (CSPT). This technique has previously been demonstrated for analyzing fluorescence from 96 wells microtiterplates (200 μl per well) and from a single cuvette with some optics to enhance sensitivity. In this work a custom designed array of wells with a volume of approximately 1 mu;l is used. In order to measure such small volumes without saturating the detector, the transmitted light is masked by placing the sample between two crossed polarizers. This arrangement blocks nearly all the transmitted light, while the emitted light, which is nearly unpolarized, can still be detected. The lowest amount (concentration x volume) of analyte detectable in this setup is about 40 times smaller than in the previous setups.

Paper Details

Date Published: 23 March 2005
PDF: 7 pages
Proc. SPIE 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III, (23 March 2005); doi: 10.1117/12.589586
Show Author Affiliations
Jimmy W. P. Bakker, Linkoeping Univ. (Sweden)
Daniel Filippini, Linkoeping Univ. (Sweden)
Ingemar Lundstroem, Linkoeping Univ. (Sweden)

Published in SPIE Proceedings Vol. 5694:
Spectral Imaging: Instrumentation, Applications, and Analysis III
Gregory H. Bearman; Anita Mahadevan-Jansen; Richard M. Levenson M.D., Editor(s)

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