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Proceedings Paper

Optical detection of Trypanosoma cruzi in blood samples for diagnosis purpose
Author(s): Elvio Alanis; Graciela Romero; Liliana Alvarez; Carlos C. Martinez; Miguel A. Basombrio
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Paper Abstract

An optical method for detection of Trypanosoma Cruzi (T. cruzi) parasites in blood samples of mice infected with Chagas disease is presented. The method is intended for use in human blood, for diagnosis purposes. A thin layer of blood infected by T. cruzi parasites, in small concentrations, is examined in an interferometric microscope in which the images of the vision field are taken by a CCD camera and temporarily stored in the memory of a host computer. The whole sample is scanned displacing the microscope plate by means of step motors driven by the computer. Several consecutive images of the same field are taken and digitally processed by means of image temporal differentiation in order to detect if a parasite is eventually present in the field. Each field of view is processed in the same fashion, until the full area of the sample is covered or until a parasite is detected, in which case an acoustical warning is activated and the corresponding image is displayed permitting the technician to corroborate the result visually. A discussion of the reliability of the method as well as a comparison with other well established techniques are presented.

Paper Details

Date Published: 21 October 2004
PDF: 5 pages
Proc. SPIE 5622, 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications, (21 October 2004); doi: 10.1117/12.589343
Show Author Affiliations
Elvio Alanis, Univ. Nacional de Salta (Argentina)
Graciela Romero, Univ. Nacional de Salta (Argentina)
Liliana Alvarez, Univ. Nacional de Salta (Argentina)
Carlos C. Martinez, Univ. Nacional de Salta (Argentina)
Miguel A. Basombrio, Univ. Nacional de Salta (Argentina)

Published in SPIE Proceedings Vol. 5622:
5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications
Aristides Marcano O.; Jose Luis Paz, Editor(s)

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