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Proceedings Paper

Use of acousto-optic tuneable filters for imaging fluorescence spectroscopy applications in vivo and in vitro
Author(s): Mounir Bouhifd; Maurice Patrick Whelan; Marc Aprahamian
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Paper Abstract

We describe the design and development two prototype spectroscopy imaging instruments based on custom-made acousto-optic tuneable filters (AOTF). These devices can be coupled to many standard imaging systems (e.g. an endoscope or a fluorescence microscope). The instruments developed offer significant advantages over typical fixed-filter imaging systems in terms of flexibility, performance and diagnostic potential. Any filtering wavelength in the visible range can be rapidly selected either by random access or continuous tuning. Since filtering is achieved through a diffractive process, an excellent signal-to-noise ratio is achieved that allows the detection of extremely low fluorescence signals such as autofluorescence. These adapters were designed to allow the simultaneous imaging of both the filtered and unfiltered signals. A first prototype instrument was developed and demonstrated for in-vivo applications. When attached to the eyepiece of a commercial endoscope, it allowed the simultaneous white light endoscopy and fluorescence imaging. Autofluorescence of protoporphyrin IX (PpIX), an endogenous chromophore that traces early-stage diseased tissue experiencing an inflammatory response, was mapped in vivo on a rat model. The system has also been approved for medical use and human clinical trials are underway. In addition, we are currently testing a second AOTF module for in vitro applications. This new AOTF adapter was designed to be coupled to the viewing port of a commercial fluorescence microscope to realise a fluorescence imaging spectrometer capable of detecting and mapping fluorescent biomolecules.

Paper Details

Date Published: 1 April 2005
PDF: 10 pages
Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); doi: 10.1117/12.589287
Show Author Affiliations
Mounir Bouhifd, Institute for Health and Consumer Protection, European Commission DG (Italy)
Maurice Patrick Whelan, Institute for Health and Consumer Protection, European Commission DG (Italy)
Marc Aprahamian, INSERM (France)

Published in SPIE Proceedings Vol. 5692:
Advanced Biomedical and Clinical Diagnostic Systems III
Tuan Vo-Dinh; Warren S. Grundfest M.D.; David A. Benaron M.D.; Gerald E. Cohn, Editor(s)

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