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Proceedings Paper

Refractive index determination from multiphoton and confocal spherical aberration microscopy
Author(s): Peter T. Fwu; Wen Lo; Chen-Yuan Dong
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Paper Abstract

Refractive index mismatch induced spherical aberration (RIMISA) is a ubiquitous problem for in-depth optical biological imaging and it is an effect most researchers like to avoid. Associated with RIMISA is a broadening of the point-spread-function (PSF) which leads to a degradation of image resolution and the loss of specimen signal. It is these features that render RIMISA to be useful for one important application in biological application, the determination of sample refractive index. We will show how RIMISA under multiphoton and confocal microscopy can be used to determine the refractive indices of uniformly luminescent specimens and a comparison will be made between the multiphoton and confocal approaches.

Paper Details

Date Published: 30 March 2005
PDF: 8 pages
Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); doi: 10.1117/12.587255
Show Author Affiliations
Peter T. Fwu, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 5700:
Multiphoton Microscopy in the Biomedical Sciences V
Ammasi Periasamy; Peter T. C. So, Editor(s)

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