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Proceedings Paper

Application of semiconductor fluorescent nanocrystals as optical probes for rapid early viral detection
Author(s): Elizabeth L. Bentzen; Frances House; Ian D. Tomlinson; Sandra J. Rosenthal; James E. Crowe; David D. Wright
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Paper Abstract

Fluorescence is a tool widely employed in biological assays. Fluorescent semiconducting nanocrystals, quantum dots (QDs), are beginning to find their way into the tool box of many biologist, chemist and biochemist. These quantum dots are an attractive alternative to the traditional organic dyes due to their broad excitation spectra, narrow emission spectra and photostability. Non-specific binding is a frequently encountered problem with fluorescent labeling in biological assays. In these studies various cell lines were examined for non-specific binding to quantum dots. Evidence suggests that non-specific binding is related to cell type and, may be significantly reduced by functionalizing quantum dots with polyethyleneglycol ligands (PEG). In addition quantum dots were used to detect and monitor the progession of the viral glycoproteins ,F (fusion) and G (attachment), from Respiratory Syncytial Virus (RSV) in HEp-2 cells. RSV is the most common cause of lower respiratory tract infection in children worldwide and the most common cause of hospitalization of infants in the US. Antiviral therapy is available for treatment of RSV but is only effective if given within the first 48 hours of infection. Existing test methods require a virus level of at least 1000-fold of the amount needed for infection of most children and require several days to weeks to obtain results. The use of quantum dots may provide an early, rapid method for detection and provide insight into the trafficking of viral proteins during the course of infection.

Paper Details

Date Published: 4 April 2005
PDF: 12 pages
Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); doi: 10.1117/12.585531
Show Author Affiliations
Elizabeth L. Bentzen, Vanderbilt Univ. (United States)
Frances House, Vanderbilt Univ. School of Medicine (United States)
Ian D. Tomlinson, Vanderbilt Univ. (United States)
Sandra J. Rosenthal, Vanderbilt Univ. (United States)
James E. Crowe, Vanderbilt Univ. School of Medicine (United States)
David D. Wright, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 5704:
Genetically Engineered and Optical Probes for Biomedical Applications III
Darryl J. Bornhop; Samuel I. Achilefu; Ramesh Raghavachari; Alexander P. Savitsky, Editor(s)

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