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Proceedings Paper

cw dye laser synchronously pumped by the third harmonic of a modelocked cw Nd:YLF laser for tunable blue and green excitation and picosecond time-correlated photon counting detection
Author(s): Arie van Hoek; Antonie J. W. G. Visser
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Paper Abstract

The range of excitation wavelengths for a setup of time-resolved detection of fluorescence was extended with tunable blue by the synchronous pumping of a Stilbene 3 dye laser. A conversion efficiency of over 30% was found when pumping a coherent radiation (CR) model CR590 with extended cavity length using the third harmonic output of a cw mode-locked Nd:YLF laser (CR Antares). For decreasing the repetition rate of excitation pulses, an electro- optic modulator in a dual pass configuration was used. In this way, pulses with durations of less than 4 ps were generated with energies of up to 2.6 nJ and a repetition rate of 600 kHz, fitting very well with the requirements of the time-correlated photon counting detection technique. The tuning range of Stilbene 3 was from 415 to 465 nm. A further extension of the wavelength range is planned by pumping Coumarin 151 (465 -520 nm) and a mixture of Stilbene 3 and Coumarin 7 (495 - 570 nm) using the same pump laser source.

Paper Details

Date Published: 1 April 1992
PDF: 5 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58281
Show Author Affiliations
Arie van Hoek, Agricultural Univ. Wageningen (Netherlands)
Antonie J. W. G. Visser, Agricultural Univ. Wageningen (Netherlands)

Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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